Systems level expression correlation of Ras GTPase regulators
نویسندگان
چکیده
منابع مشابه
Epidermal growth factor increases protein and messenger RNA expression levels of Ras GTPase activating protein.
The Ras GTPase activating protein (RasGAP) is a key regulatory enzyme in the Ras signaling pathway, which is crucial for growth factor-induced mitogenesis. In this study, it is shown that epidermal growth factor (EGF) increases RasGAP protein expression by 100-150% in NIH 3T3 cells, which overexpress the human EGF receptor, and mouse keratinocytes but does not increase RasGAP protein expression...
متن کاملExpression of GTPase-deficient Ras inhibits vasopressin signaling in cultured cortical collecting duct cells.
Cross-talk between signaling pathways is increasingly recognized as integral to cellular function. We investigated whether the mitogen-activated protein kinase (MAPK) pathway alters vasopressin (AVP) stimulation of protein kinase A (PKA) by specifically studying the role of Ras. Mouse cortical collecting duct cells (M-1) were transfected with a cDNA encoding oncogenic Ras. Transfection was conf...
متن کاملHa-ras proteins exhibit GTPase activity: point mutations that activate Ha-ras gene products result in decreased GTPase activity.
Several ras genes have been expressed at high levels in Escherichia coli and the resultant ras proteins were shown to be functional with respect to their well-known specific, high-affinity, GDP/GTP binding. We were able to detect a weak GTPase activity associated with the purified proteins. The normal cellular ras protein (p21N) exhibits approximately equal to 10 times higher GTPase activity th...
متن کاملKinetic and cell-based analyses of GTPase regulators
Regulatory GTPases are often portrayed as binary molecular switches that control a wide range of cellular processes, including, but not limited to, the generation of second messengers (e.g., cAMP and inositol phosphates), intracellular traffic, cytoskeleton organization and cell proliferation. GEF stimulators and GAP inhibitors regulate the nucleotide-bound state of these proteins. Because of t...
متن کاملEffect of ligands on stability of H-Ras GTPase
The G domain of a small monomeric GTPase Ras contains a nucleotide-binding pocket and a magnesiumbinding site essential for the Ras function in cellular signaling. The G domain also has another (allosteric) ion-binding site on the rear surface of the G domain, which function is still unknown. In this paper, we detailed the effect of calcium and magnesium ions on stability of Ras bound to GDP, G...
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ژورنال
عنوان ژورنال: Cell Communication and Signaling
سال: 2018
ISSN: 1478-811X
DOI: 10.1186/s12964-018-0256-8